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cripto-1-higg-fc gene  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cripto-1-higg-fc gene
    Cripto 1 Higg Fc Gene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cripto/pm39840816-294-1-7
    Average 90 stars, based on 1 article reviews
    cripto-1-higg-fc gene - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Over Expression:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Amplification:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Clone Assay:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Expressing:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Construct:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Purification:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Sequencing:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Modification:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Affinity Chromatography:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Size-exclusion Chromatography:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Molecular Weight:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    SDS Page:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Ligand Binding Assay:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Injection:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Binding Assay:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Concentration Assay:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Inhibition:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Luciferase:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Western Blot:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Transfection:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Plasmid Preparation:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Reporter Assay:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    shRNA:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Cell Differentiation:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Cell Culture:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1


    Real-time Polymerase Chain Reaction:

    Article Title: Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization.
    Article Snippet: Funding information Australian Research Council Summary Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations.. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation.. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 30 end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele.

    Article Title: Cripto‐1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains
    Article Snippet: A synthetic Cripto‐1‐hIgG‐Fc gene was obtained from GeneArt.

    Article Title: Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic
    Article Snippet: Expression Plasmids Synthetic Cripto-1-hIgg-Fc and cryptic-hIgg-Fc genes were obtained from GeneArt.

    Article Title: Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway
    Article Snippet: For overexpression study, Cripto-1 coding region was amplified with primers indicated at Supplementary Table and was cloned into pcDNA3.1 (Cripto-1-OE) (Invitrogen).

    Article Title: Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains.
    Article Snippet: A synthetic Cripto-1-hIgG-Fc gene was obtained from GeneArt.

    Article Title: Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Article Snippet: Taqman probe, Mouse Gapdh Applied Biosystems Mm99999915_g1 Taqman probe, Mouse Pou5f1 (Oct4) Applied Biosystems Mm03053917_g1 Taqman probe, Mouse Nanog Applied Biosystems Mm02019550_s1 Taqman probe, Mouse Troma-I (Krt8) Applied Biosystems Mm04209403_g1 Taqman probe, Mouse Atp1b1 Applied Biosystems Mm00437612_m1 Taqman probe, Mouse Cdx2 Applied Biosystems Mm01212280_m1 Taqman probe, Mouse Bmp4 Applied Biosystems Mm00432087_m1 Taqman probe, Mouse Lifr Applied Biosystems Mm00442942_m1 Taqman probe, Mouse Gata3 Applied Biosystems Mm00484683_m1 Taqman probe, Mouse Prdm14 Applied Biosystems Mm01237814_m1 Taqman probe, Mouse Prdm1 (Blimp1) Applied Biosystems Mm00476128_m1 Taqman probe, Mouse Id1 Applied Biosystems Mm00775963_g1 Taqman probe, Mouse Id2 Applied Biosystems Mm00711781_m1 Taqman probe, Mouse Id3 Applied Biosystems Mm00492575_m1 Taqman probe, Mouse Id4 Applied Biosystems Mm00499701_m1 Taqman probe, Mouse Zscan4 Applied Biosystems Mm02581232_m1 Taqman probe, Mouse Eomes Applied Biosystems Mm01351984_m1 Taqman probe, Mouse Tdgf1 (Cripto) Applied Biosystems Mm03024051_g1 Taqman probe, Mouse Cdh1 (Ecadherin) Applied Biosystems Mm01247357_m1 Taqman probe, Mouse Gata4 Applied Biosystems Mm00484689_m1 Taqman probe, Mouse Sox2 Applied Biosystems Mm03053810_s1 Taqman probe, Mouse Sox17 Applied Biosystems Mm00488363_m1 Taqman probe, Mouse Zfp42 (Rex1) Applied Biosystems Mm03053975_g1 Taqman probe, Mouse Tcstv3 Applied Biosystems Mm02025498_s1




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